RNA

Part:BBa_I1060:Design

Designed by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton   Group: Antiquity   (2003-01-31)


Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense inhibition)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_I1060 Custom cI Protein with LacO-1 Promoter is based on BioBricks BBa_C0050 and BBa_R0011. It is designed such that BBa_I1063 can be used to interfere with the BBa_1060 transcript by anti-sense mRNA binding, in the IS10 method shown below. BBa_I1060 and BBa_I1063 can act indepently of related biobrick parts BBa_1010, BBa_1011, BBa_1012, BBa_I1030, BBa_I1031, BBa_I1032, BBa_I1041, BBa_I1042, BBa_I1020, BBa_I1023 and BBa_1013.

Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1063. Incompatible with systems containing cI (wild type)



Source

Modified from BioBricks BBa_R0011, and BBa_C0051.

References